Evaluation of a Dye-binding Method for Determination of Serum Albumin.
نویسنده
چکیده
A simple dye-binding method for determining serum albumin, which employs the dye, 2-(4’-hydroxybenzeneazo) benzoic acid (HBABA), is described. Albumin in 0.1 ml. of serum is determined colorimetrically at 510 m s. Reliability of the method has been established by comparison with another method using HBABA and with a salt fractionation-biuret method, by recovery experiments, variation in ambient temperature, demonstration of optimal dye molarity and pH, and replicate analyses.
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عنوان ژورنال:
- Clinical chemistry
دوره 11 شماره
صفحات -
تاریخ انتشار 1965